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Mycobiota of energy sorrel
Janďourková, Hana ; Novotný, David (advisor) ; Mazáková, Jana (referee)
Energy sorrel (Rumex patientia L. x Rumex tianschanicus A. Los.) is a plant with a high yield of above-ground biomass. Because of this, it is grown as energy crop, but in Ukraine it is also important forage crop. Mycobiota has not been examined, even though energy sorrel is grown in the Czech Republic experimentally since 1992 (in agricultural operations since 2001). The aims of this thesis were: to determine the composition of mycobiota of healthy plants and of mycobiota associated with leaf and stem spots, to verify whether fungi isolated from spots can cause these spots and if previously isolated fungus Phomopsis sp. can cause spots. Samples were colected from 4 sites in Czech republic, in three samplings during the vegetation season. Endophytes were isolated from root, stem and leaf. Fungi asociated with spots were isolated from stem and leaf. Fungi were cultivated on 2% malt extract agar and then morphologically determined. From representative strains were isolated DNA and sequences of the ITS region were compared with the GenBank databases, this verified the morphological determination and the sterile species were determined. The most common endophytes were Clonostachys rosea f. rosea, Cladosporium cladosporioides, Rhizopus arrhizus var. arrhizus, Alternaria alternata a Acremonium strictum...
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Mycobiota of energy sorrel
Janďourková, Hana ; Novotný, David (advisor) ; Mazáková, Jana (referee)
Energy sorrel (Rumex patientia L. x Rumex tianschanicus A. Los.) is a plant with a high yield of above-ground biomass. Because of this, it is grown as energy crop, but in Ukraine it is also important forage crop. Mycobiota has not been examined, even though energy sorrel is grown in the Czech Republic experimentally since 1992 (in agricultural operations since 2001). The aims of this thesis were: to determine the composition of mycobiota of healthy plants and of mycobiota associated with leaf and stem spots, to verify whether fungi isolated from spots can cause these spots and if previously isolated fungus Phomopsis sp. can cause spots. Samples were colected from 4 sites in Czech republic, in three samplings during the vegetation season. Endophytes were isolated from root, stem and leaf. Fungi asociated with spots were isolated from stem and leaf. Fungi were cultivated on 2% malt extract agar and then morphologically determined. From representative strains were isolated DNA and sequences of the ITS region were compared with the GenBank databases, this verified the morphological determination and the sterile species were determined. The most common endophytes were Clonostachys rosea f. rosea, Cladosporium cladosporioides, Rhizopus arrhizus var. arrhizus, Alternaria alternata a Acremonium strictum...
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Mycobiota of energy sorrel
Janďourková, Hana ; Novotný, David (advisor) ; Nováková, Alena (referee)
Energy sorrel (Rumex patientia L. x Rumex tianschanicus A. Los.) is a plant with a high yield of above-ground biomass. Because of this, it is grown as energy crop, but in Ukraine it is also important forage crop. Mycobiota has not been examined, even though energy sorrel is grown in the Czech Republic experimentally since 1992 (in agricultural operations since 2001). The aims of this thesis were: to determine the composition of mycobiota of healthy plants and of mycobiota associated with leaf and stem spots, to verify whether fungi isolated from spots can cause these spots and if previously isolated fungus Phomopsis sp. can cause spots. Fungi were cultivated on 2% malt extract agar and then morphologically determined. From representative strains were isolated DNA and sequences of the ITS region were compared with the GenBank databases, this verified the morphological determination and the sterile species were determined. In total 24 morphotypes of endophytic fungi were cultivated from healthy plants. The most common species were: Clonostachys rosea f. rosea, Cladosporium cladosporioides, Rhizopus arrhizus var. arrhizus, Alternaria alternata and Acremonium strictum. From the spots were isolated in total 29 morphotypes, the dominant species were: A. strictum, Aureobasidium pullulans, C....
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